Histone H2B Deacylation Selectivity: Exploring Chromatin's Dark Matter with an Engineered Sortase.

We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and ß-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics.

Concurrent phone texting alters crossing behavior and induces gait imbalance during obstacle crossing.

Texting during walking has become a very common daily activity and could alter gait performance, especially during locomotion when additional visual attention is demanded, such as obstacle crossing. The purpose of this study was to examine biomechanical changes in obstructed gait characteristics while engaging in a phone texting activity. Gait analyses were performed on ten young healthy adults under the following two tasks: 1) walking and crossing an obstacle set at a 10% of the subject's height and 2) walking and crossing an obstacle while responding to a text message. Whole body motion data were collected with a 10-camera motion capture system. Our data demonstrated that a conservative gait pattern was adopted while performing texting when approaching and crossing over the obstacle, which was indicated by slower walking speeds and greater toe-obstacle clearances. This gait pattern was, however, accompanied by a greater body sway in the frontal plane during crossing, which could be an indication of perturbed gait balance control. Increased visual-attentional demand from a concurrent phone texting could negatively impact young pedestrians' safety during obstacle crossing.